D4013 DNA纯化试剂盒-5

DNA Clean & Concentrator™-5，DNA纯化试剂盒-5

DNA Clean & Concentrator™-5，DNA纯化试剂盒-5
DNA Clean & Concentrator™-5，DNA纯化试剂盒-5
DNA Clean & Concentrator™-5，DNA纯化试剂盒-5

DNA Clean & Concentrator™-5

Clean and concentrate up to 5 μg DNA with ≥ 6 μl elution in as little as 2 minutes with 0 μl wash residue carryover.

Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer.

Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.

about DNA Clean & Concentrator™-5

The DNA Clean & Concentrator™-5 product provides purification of up to 5 µg DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc. This product facilitate the removal of DNA polymerases, modifying enzymes, RNA polymerases, ligases, kinases, nucleases, phosphatases, and restriction endonucleases, as well as free dNTPs and their analogs including radiolabeled and fluorescent derivatives. Eluted DNA is suitable for PCR, arrays, ligation, sequencing, etc.

Specifications

Format: Spin Column (capped/uncapped)

DNA Recovery: Typically, ≤5 µg total DNA can be eluted with ≥6 µl water or low salt elution buffer. For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%.

Equipment: Microcentrifuge

DNA Size Limits: 50 bp to 23 kb

Sample Sources: DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc.

DNA Purity: Highly purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions.

Product Detergent Tolerance: ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS

Protocol

Step 1: In a 1.5 ml microcentrifuge tube, add 2-7 volumes of DNA Binding Buffer to each volume of DNA sample (see table below). Mix briefly by vortexing.

Application	DNA Binding Buffer : Sample	Example
Plasmid, genomic DNA (>2 kb)	2: 1	200 µl : 100 µl
PCR product, DNA fragment	5: 1	500 µl : 100 µl
ssDNA (e.g. cDNA, M13 phage)	7: 1	700 µl : 100 µl

For efficient recovery of genomic or large DNA (< 20 kb to < 200 kb), use the Genomic DNA Clean & Concentrator™ (Cat. Nos. D4010, D4011).

Step 2: Transfer mixture to a provided Zymo-Spin™ Column in a Collection Tube.

Step 3: Centrifuge for 30 seconds.  Discard the flow-through.

Step 4: Add 200 µl DNA Wash Buffer to the column.  Centrifuge for 30 seconds.  Repeat the wash step.

Step 5: Add ≥ 6 µl DNA Elution Buffer or water directly to the column matrix and incubate at room temperature for one minute.  Transfer the column to a 1.5 ml microcentrifuge tube and centrifuge for 30 seconds to elute the DNA.

Ultra-pure DNA is now ready for use.

Product Contents

DNA Clean & Concentrator™-5(Kit Size)	D4013(50 Preps.)	Storage Temperature
DNA Binding Buffer	50 ml	Room Temp.
DNA Wash Buffer	6 ml	Room Temp.
DNA Elution Buffer	1 ml	Room Temp.
Zymo-Spin™ I Columns	50 (D4013 – capped columns)	Room Temp.
Collection Tubes	50	Room Temp.
Instruction Manual	1	Room Temp.

D4013-DNA Clean & Concentrator™-5-Data Sheet
D4013-DNA Clean & Concentrator™-5-MSDS
D4013-DNA Clean & Concentrator™-5-Protocol


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