tlrl-hkmt-5 HKMT (无内毒素污染，生物学活性检测，无菌）

Heat-killed Mycobacterium tuberculosis

HKMT is a heat-killed preparation of the avirulent strain of Mycobacterium tuberculosis H37 Ra.

HKMT is sensed by Mincle which recognizes the mycobacterial cell wall glycolipid TDM. HKMT also possesses a large repertoire of TLR2 ligands, such as lipoproteins and lipomannan. Upon HKMT sensing, both Mincle and TLR2 lead to the activation of NF-κB.

1. Ishikawa E. et al., 2009. Direct recognition of the mycobacterial glycolipid, trehalose dimycolate, by C-type lectin Mincle. 206(13):2879-88.
2. Bhatt K & Salgame P., 2007. Host innate immune response to Mycobacterium tuberculosis. J Clin Immunol 27(4): 347–362.

Specifications

Specificity: Mincle agonist, TLR2 agonist

Working concentration:

• 10 - 100 µg/ml when used as a Mincle ligand
• 100 ng - 10 µg/ml when used as a TLR2 ligand

Contents

Heat-killed preparation of Mycobacterium tuberculosis H37Ra (HKMT) is provided lyophilized and is available in two quantities:

tlrl-hkmt-1:

• 10 mg lyophilized HKMT
• 1.5 ml endotoxin-free water

tlrl-hkmt-5:

• 5 x 10 mg lyophilized HKMT
• 10 ml endotoxin-free water

HKMT is shipped at room temperature

Stored at 4˚C.

Citations

2016 Sci Signal. 9(449):ra101.

Lipoarabinomannan binding to lactosylceramide in lipid rafts is essential for the phagocytosis of mycobacteria by human neutrophils.

Nakayama H. et al.

2016 Biochem J. 473(18):2845-61.

TLR and TNF-R1 activation of the MKK3/MKK6-p38α axis in macrophages is mediated by TPL-2 kinase.

Pattison MJ. et al.