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价格:电议
所在地:上海
型号:91-200-88
更新时间:2016-12-06
浏览次数:7629
公司地址:上海市闵行区都庄路4226号 福克斯商务大厦3幢201室
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颜敏(先生)
红荣微再(上海)生物工程技术有限公司,作为专业的细胞培养产品供应商,非常荣幸的获得Corning康宁公司授权在华东区域浙江,安徽,湖北的授权代理商,授权销售其在免疫治疗领域的相关产品,包括诸如细胞冻存袋,无血清淋巴细胞培养基,无血清细胞培养袋等。欢迎新老用户前来咨询洽谈。红荣微再(上海)生物工程技术有限公司将一如既往的为您提供高品质细胞培养产品和配套技术服务。
康宁的一次性使用的冻存袋配有专用聚烯烃/ EVA(乙烯醋酸乙烯酯)膜融合。这种独特的薄膜袋容器保持在超低温柔性。康宁的一套低温袋设计的贮藏,保鲜和细胞转移。推荐的填充体积为20毫升至190毫升的四种标准尺寸。冻结体积建议是基于在具有大约0.313英寸内部厚度的保护盒水平冻结。
特征
wRemains灵活的在超低温(-196℃)
当充满液体wMaintains清晰
wMembrane端口设计提供了壁更薄,以提高灵活性
wAttached帽冷冻过程中zui大限度地减少膜曝光
wStandard标签口袋提供了标签更容易使用的和可追溯
wUsed基础研究和生物制药应用
wAvailable以下尺寸:50毫升,250毫升,500毫升,750毫升
使用说明
康宁冻存袋的贮藏,保鲜和细胞的液氮转移到-196℃。冷冻保存袋无菌提供和供一次性使用的。
建议的冷冻量是基于在具有大约8mm的-纳尔间厚度的保护盒水平冻结。如果容器被垂直冻结或具有不同厚度的盒,用户必须确定填充体积。
Corning®样品冷却和加热解决方案提供高度一致、可重复和标准化的温度控制方法。我们提供的方案适合有冰、无冰、外接电源或用电池等多种使用模式。为您减少意料之外的温度失控,降低污染风险,使您的样品始终处于您所要求的温度条件。
将样品管、板或孔安置于Corning CoolRack® 和CoolSink® 热传导模块,使用冰,干冰,酒精浴,水浴锅等冷却源或加热源,可以减少由于样品直接置于冷却源或加热源上而造成的样品间温度差异。
我们提供的解决方案灵活多样,您可以根据实验需求选择合适的配件组成。CoolRack和CoolSink模块既放置在传统冰桶、冰盘,又可以结合无冰冷却系统使用,如Corning CoolBox™产品。无论您选择何种组合方式,我们都可以确保您的样品温度稳定,保证您的研究从开始的一步就顺利进行。
欢迎您致电 华雅再生医学旗舰公司:红荣微再(上海)生物工程技术有限公司 号:。华雅再生医学-客服
Ordering Information
|
Cat. No. |
Description |
Film Type |
Unit Size |
Fill Volume |
Qty |
|
91-200-88 |
Cryopreservation Bag |
Polyolefin/EVA |
50 mL |
10-20 mL |
1 |
|
91-200-89 |
Cryopreservation Bag |
Polyolefin/EVA |
250 mL |
30-70 mL |
1 |
|
91-200-90 |
Cryopreservation Bag |
Polyolefin/EVA |
500 mL |
55-100 mL |
1 |
|
91-200-91 |
Cryopreservation Bag |
Polyolefin/EVA |
750 mL |
80-190 mL |
1 |
Freezing Precautions
•Use of a protective storage cassette is strongly recommended during frozen storage.
•Ensure bag exterior and protective freezing cassettes are dry prior to initiating freezing protocol. Moisture on the exterior of the bag or on the cassette could cause adherence of the bag to the cassette resulting in difficulty of bag removal.
•When using controlled rate freezing, maintain a consistent freezing profile.
•After freezing, do not handle excessively. Port tubes and film are fragile in the frozen state and breakage may occur.
Thawing Recommendations
•Use of an overwrap is strongly recommended during thawing.
•Harvest cells as soon as possible after thawing. DMSO can be toxic at room temperature.
Instructions
Use aseptic technique
1.Remove bag from protective pouch. Assure all adapter covers are secure. Do not use if adapter cov-ers are not in place.
2.Close all clamps on transfer set, if needed.
3.Remove protective caps from distal end of transfer set as needed and attach to transfer tubing.
4.Do not over tighten luers. Hand tighten only.
5.Initiate aseptic transfer by opening pinch clamp of appropriate tubing segment.
6.Remove as much air as possible from the container.
7.Seal tubing between base connection of transfer set and bag shoulder, using dielectric sealer, or equivalent. Bag should be flat and supported during sealing.
8.Examine seal to assure weld process is complete. If weld is incomplete, another seal may be initiated to a lower segment of the tubing closer to the bag shoulder.
9.Remove and discard the transfer set by cutting the tubing above the seal.
10.Insert label in label pocket, apply multiple spot seals to pocket using dielectric sealer, or equivalent. Do not seal entire open edge of label pocket.
11.Place bag in freezing cassette and follow freezing protocol.
Instructions (Thawing)
1.Remove product from liquid nitrogen storage tank.
2.Remove product from freezing cassette. If bag is affixed to cassette, warm the bag by exposing to ambient temperatures for 1 minute. Repeat this step if the bag remains affixed to the cassette.
3.Place frozen product in a suitable overwrap bag to ensure waterbath is not in direct contact with freezing bag.
4.Thaw in water bath with gentle agitation at 37 - 40°C.
5.The product is completely thawed when the last ice crystal is melted.
欢迎您致电 华雅再生医学旗舰公司:红荣微再(上海)生物工程技术有限公司 号:。华雅再生医学-客服
免责声明:以上所展示的[91-200-88 Corning康宁细胞冻存袋]信息由会员[红荣微再(上海)生物工程技术有限公司]自行提供,内容的真实性、准确性和合法性由发布会员负责。