文献：泛素交联酶抗体应用方法

中文名称    泛素交联酶抗体    

英文名称    E2EPF    

别    名    E2 EPF; E2EPF; EPF5; UBE2S; Ubiquitin carrier protein S; Ubiquitin conjugating enzyme E2 24 kD; Ubiquitin conjugating enzyme E2S; Ubiquitin protein ligase S; AA409170; MGC101982; UBE2S_HUMAN.    

供 应 商    远慕生物  

研究领域    免疫学  转录调节因子      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 泛素交联酶抗体（石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    24kDa    

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human E2EPF    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

泛素交联酶抗体产品介绍    background:

This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. [provided by RefSeq, Jul 2008]

Function:
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as a E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination.

Subunit:
Component of the APC/C complex. Interacts with CDC20, FZR1/CDH1 and VHL.

Post-translational modifications:
Autoubiquitinated by the APC/C complex during G1, leading to its degradation by the proteasome.

Similarity:
Belongs to the ubiquitin-conjugating enzyme family.

Gene ID:
27338

Database links:

Entrez Gene: 617703 Cow

Entrez Gene: 27338 Human

Entrez Gene: 77891 Mouse

Entrez Gene: 292588 Rat

GenBank: NP_055316.2 Human

Omim: 610309 Human

SwissProt: Q1RML1 Cow

SwissProt: Q16763 Human

SwissProt: Q921J4 Mouse

SwissProt: B5DFI8 Rat

Unigene: 396393 Human

Unigene: 389787 Mouse

Unigene: 120398 Rat


important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.