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ab172730 兔IgG，单克隆抗体[EPR2]

北京优尼康生物科技有限公司

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价格：电议

所在地：北京

型号：ab172730

更新时间：2023-08-11

浏览次数：986

公司地址：北京市顺义区府前街56号院1号楼4层1-416

18618266805/13911868362 赵先生(先生) 销售业务员

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产品简介

兔IgG，单克隆抗体[EPR2]

公司简介

北京优尼康生物科技有限公司主要经营进口、国产生物科研仪器、试剂及耗材，提供技术服务的生物公司之一。现经营的品牌及相关产品如下：

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产品说明

兔IgG，单克隆抗体[EPR2]
兔IgG，单克隆抗体[EPR2]
兔IgG，单克隆抗体[EPR2]

概述

产品名称

兔IgG，单克隆抗体[EPR2] -同型对照
经测试应用

适用于: ICC/IF, Flow Cyt, IHC-P, CHIPseq, IP
常规说明

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents

We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

This product is a recombinant rabbit monoclonal antibody.

性能

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
浓度

1.775 mg/ml
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR2
同种型

IgG

应用

Our Abpromise guarantee covers the use of ab172730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用	Ab评论	说明
ICC/IF		Use at an assay dependent concentration. Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.
Flow Cyt		Use at an assay dependent concentration. Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.
IHC-P		Use at an assay dependent concentration. Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.
CHIPseq		Use at an assay dependent concentration. PubMed: 26455392
IP		Use at an assay dependent concentration.

图片

Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal - Isotype Control (ab172730)

Immunofluorescent staining of HeLa cells using anti-AIF RabMAb (ab32516, left panel) (green) and Rabbit mAb IgG control (ab172730, right panel). DAPI nuclear staining (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal - Isotype Control (ab172730)

Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Vimentin RabMAb (ab92547, left panel) (brown) and Rabbit mAb IgG control (ab172730, right panel).
Flow Cytometry - Rabbit IgG, monoclonal [EPR2] - Isotype Control (ab172730)

Overlay histogram showing A549 (human lung carcinoma) cells stained with ab133557 (red line). The cells were fixed with 2% paraformaldehyde. The cells were then incubated with ab133557 at 1/60 dilution. The secondary antibody used was goat anti-rabbit IgG (FITC) at 1/150 dilution. Isotype control antibody (black line) was rabbit IgG (monoclonal) (ab172730). Unlabelled control (blue line) was cells without incubation with primary antibody and secondary antibody. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 530/30 bandpass filter.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal - Isotype Control (ab172730)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Vimentin RabMAb (ab92547, left panel) (brown) and Rabbit mAb IgG control (ab172730, right panel).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [EPR2] - Isotype Control (ab172730)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue with unpurified Rabbit IgG ab172730 at 1/10. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [EPR2] - Isotype Control (ab172730)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue with purified Rabbit IgG ab172730 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [EPR2] - Isotype Control (ab172730)

Immunocytochemsitry/Immunofluorescence analysis of HeLa cells with purified Rabbit IgG ab172730 at 1/100. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).
Immunocytochemistry/ Immunofluorescence - Rabbit IgG, monoclonal [EPR2] - Isotype Control (ab172730)

Immunocytochemsitry/Immunofluorescence analysis of HeLa cells with unpurified Rabbit IgG ab172730 at 1/10. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^®488-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).
Flow Cytometry - Rabbit IgG, monoclonal [EPR2] - Isotype Control (ab172730)

Overlay histogram showing K562 (human chronic myelogenous leukemia) cells stained with ab196018 (red line). The cells were fixed with 2% paraformaldehyde. The cells were then incubated with ab196018 at 1/150 dilution. The secondary antibody used was goat anti-rabbit IgG (FITC) at 1/150 dilution. Isotype control antibody (black line) was rabbit IgG (monoclonal) (ab172730). Unlabelled control (blue line) was cells without incubation with primary antibody and secondary antibody. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 530/30 bandpass filter.
Flow Cytometry - Rabbit IgG, monoclonal [EPR2] - Isotype Control (ab172730)

Overlay histogram showing SH-SY5Y (human neuroblastoma) cells stained with ab179513 (red line). The cells were fixed with 2% paraformaldehyde. The cells were then incubated with ab179513 at 1/150 dilution. The secondary antibody used was goat anti-rabbit IgG (FITC) at 1/150 dilution. Isotype control antibody (black line) was rabbit IgG (monoclonal) (ab172730). Unlabelled control (blue line) was cells without incubation with primary antibody and secondary antibody. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 530/30 bandpass filter.
Flow Cytometry - Rabbit IgG, monoclonal [EPR2] - Isotype Control (ab172730)

Overlay histogram showing A549 (human lung carcinoma) cells stained with ab185633 (red line). The cells were fixed with 2% paraformaldehyde. The cells were then incubated with ab185633 at 1/150 dilution. The secondary antibody used was goat anti-rabbit IgG (FITC) at 1/150 dilution. Isotype control antibody (black line) was rabbit IgG (monoclonal) (ab172730). Unlabelled control (blue line) was cells without incubation with primary antibody and secondary antibody. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 530/30 bandpass filter

文献

This product has been referenced in:

Xu JG et al. TGF-ß1-induced differentiation of SHED into functional smooth muscle cells. Stem Cell Res Ther 8:10 (2017). Flow Cyt . Read more (PubMed: 28114966) »
Cannon C et al. Therapeutic targeting of protein kinase CK2 gene expression in feline oral squamous cell carcinoma - a naturally occurring large animal model of head and neck cancer. Hum Gene Ther Clin Dev N/A:N/A (2017). IHC . Read more (PubMed: 28335614) »

See all 35 Publications for this product

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Datasheet
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