多色预染低分子量蛋白质标准
多色预染低分子量蛋白质标准
多色预染低分子量蛋白质标准
描述
Thermo Scientific Spectra Multicolor Low Range Protein Ladder is a mixture of six proteins and synthetic peptides (1.7 to 40kDa) for use as size standards for peptides and small proteins in gel electrophoresis and Western blotting.
规格
Stain Type:
|
Prestained
|
System:
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SDS-PAGE
|
Product Line:
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Spectra™
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Product Size:
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250 µL
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内容及储存
Upon receipt store at -20°C. Product is shipped with an ice pack.
Features of Spectra Multicolor Low Range Protein Ladder:
• Size range—six proteins and peptides spanning 1.7 to 40kDa
• Multicolor—three different colors for unambiguous band-size assignment
• Ready-to-use—supplied in a loading buffer for direct loading on gels; no need to boil
• Sharp bands—color-coded bands of similar intensity for easy visualization
• Quality tested—each lot e
valuated by SDS-PAGE and Western blotting
• Membrane-compatible—colored bands transfer to membranes for Western blotting
Includes:
• Dye-stained proteins in 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol.
Applications:
• Mo
nitoring of protein migration during SDS-PAGE
• Verifying Western transfer efficiency
• Approximate sizing of proteins on SDS-polyacrylamide gels and Western blots
• Locating a protein of interest for excision from an unstained preparative gel
This prestained protein MW marker is designed for mo
nitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency o
nto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. Three different chromophores (blue, orange, green) are bound to the different compo
nent proteins, producing a brightly colored ladder with an easy-to-remember pattern. The Spectra Multicolor High Range Protein Ladder is ready to use: no heating, further dilution or addition of a reducing agent is required.
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