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34095 超敏化学发光底物

北京优尼康生物科技有限公司

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价格：电议

所在地：北京

型号：34095

更新时间：2023-08-11

浏览次数：892

公司地址：北京市顺义区府前街56号院1号楼4层1-416

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产品简介

超敏化学发光底物

公司简介

北京优尼康生物科技有限公司主要经营进口、国产生物科研仪器、试剂及耗材，提供技术服务的生物公司之一。现经营的品牌及相关产品如下：

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产品说明

超敏化学发光底物
超敏化学发光底物
超敏化学发光底物

Description Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for low-femtogram-level detection by Western blot analysis with horseradish peroxidase (HRP) enzyme.
Features of SuperSignal West Femto Maximum Sensitivity Substrate:
• ECL —enhanced chemiluminescent substrate for horseradish peroxidase (HRP)
• Sensitive—detect low-femtogram (mid-zeptomole) amounts of protein in bands on nitrocellulose or PVDF membranes when probed with appropriate primary and secondary antibodies
• Quantitative—produces quantitative signal that is measurable over two orders of magnitude
• Intense signal—easy to capture an image by exposure to film or imaging system
• Excellent signal duration—8 hours of usable light-output when conditions are optimized
• Stable reagent—kit components are stable for 1 year at 4°C or 6 months at room temperature
• Economical—optimized for extremely dilute antibody concentrations:
• 1ng to 0.2 µg/mL primary antibody (1:5000 to 1:100,000 dilution from 1 mg/mL stock)
• 2 to 10 ng/mL secondary antibody (1:100,000 to 1:500,000 dilution from 1 mg/mL stock)
When combined with optimized antibody concentrations and blocking buffers, SuperSignal West Femto Substrate enables detection of target proteins in amounts that are too small to be seen with typical ECL substrates.

For Research Use Only. Not for use in diagnostic procedures.

Figures

Purified IκB was serially diluted from 100 to 1fg and then electrophoresed on a 4-20% mini gel. The protein was transferred to PVDF membrane and blocked withStartingBlock Blocking Buffer for 1 ho

NIH3T3 lysate was diluted in electrophoresis reducing sample buffer. Lane 1 co<ems></ems>ntained 5µg of NIH3T3 lysate. Five 1:1 serial dilutions were then prepared and applied at 10μL/well. After electr

NIH3T3 lysate was diluted in electrophoresis reducing sample buffer. Lane 1 contained 5µg of NIH3T3 lysate. Five 1:1 serial dilutions were then prepared and applied at 10μL/well. After electr

A431cell lysate was diluted in electrophoresis reducing sample buffer at 5, 2.5, 1.25, 0.625, 0.3125, and 0.15625µg/well with a 10µL/well load. After electrophoresis, proteins were transfe

Specifications

Detection Method:	Chemiluminescent
Product Line:	SuperSignal™
Product Size:	100 mL
Substrate Properties:	Chemical Substrate
Substrate Type:	HRP (Horseradish Peroxidase) Substrate