索引：磷酸化驱动蛋白样蛋白1抗体应用方法_应用方法_资料

中文名称    磷酸化驱动蛋白样蛋白1抗体

英文名称    phospho-Eg5(Thr926)

别    名    Eg5 (phospho T926); KIF11(phospho T926); TRIP5(phospho T926); p-Eg5(Thr926); p-KIF11(Thr926); p-TRIP5(Thr926); HKSP; KIF11; kinesin family member 11; Kinesin like protein 1; Kinesin like spindle protein HKSP; KNSL1; Thyroid receptor interacting protein 5; TRIP5; KIF11_HUMAN.

供应商    远慕生物

产品类型    磷酸化抗体

研究领域    细胞生物  信号转导  细胞骨架  表观遗传学

抗体来源    Rabbit

克隆类型    Polyclonal

交叉反应    Human, Chicken, Cow, Horse,

产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 磷酸化驱动蛋白样蛋白1抗体（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分子量    120kDa

细胞定位    细胞浆

性    状    Lyophilized or Liquid

浓    度    1mg/1ml

免疫原    KLH conjugated Synthesised phosphopeptide derived from human Eg5 around the phosphorylation site of Thr926 [GT(p-T)PQ]

亚    型    IgG

纯化方法    affinity purified by Protein A

储存液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

磷酸化驱动蛋白样蛋白1抗体产品介绍    background:

Eg5 is a motor protein that belongs to the kinesin-like family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis.

Function:
Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.

Subunit:
Interacts with the thyroid hormone receptor in the presence of thyroid hormone. Component of a large chromatin remodeling complex, at least composed of MYSM1, PCAF, RBM10 and KIF11/TRIP5. Interacts (via C-terminus) with the kinase NEK6 in both interphase and mitosis.

Subcellular Location:
Cytoplasm. Cytoplasm, cytoskeleton, spindle pole.

Post-translational modifications:
Phosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase). Phosphorylated upon DNA damage, probably by ATM or ATR.
A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function.

Similarity:
Belongs to the kinesin-like protein family. BimC subfamily.
Contains 1 kinesin-motor domain.

Database links:
UniProtKB/Swiss-Prot: P52732.2

important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.