文献：嗜酸性粒细胞过氧化物酶抗体应用方法

中文名称    嗜酸性粒细胞过氧化物酶抗体    

英文名称    EPX    

别    名    Eosinophil peroxidase; Eosinophil peroxidase heavy chain; EPER; EPO; EPP; EPX PEN; PERE; PERE_HUMAN.    

供 应 商    远慕生物

研究领域    肿瘤  免疫学      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 嗜酸性粒细胞过氧化物酶抗体（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    79kDa    

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human Eosinophil peroxidase heavy chain    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

嗜酸性粒细胞过氧化物酶抗体产品介绍    background:

EPX, eosinophil peroxidase, is an antimycobacterial protein that localizes to cytoplasmic granules of eosinophils and recruits bromide to generate a halogenating oxidant. EPX dependent generation of hypobromous acid causes damage to tissue during inflammatory conditions that include asthma, allergies,cancer and parasitic/helminthic infections. EPX is a major enzyme present in eosinophils and upon degranulation, becomes released into the airways of asthmatics. As a result of its cationic nature and its ability to catalyze the formation of highly toxic oxidants, EPX can induce lung injury in a JNK dependent manner.

Function:
Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. Shows significant inhibitory activity towards Mycobacterium tuberculosis H37Rv by inducing bacterial fragmentation and lysis.

Subunit:
Tetramer of two light chains and two heavy chains.

Subcellular Location:
Cytoplasmic granule. Note=Cytoplasmic granules of eosinophils.

DISEASE:
Defects in EPX are the cause of eosinophil peroxidase deficiency (EPD) [MIM:261500]. EPD is an autosomal recessive defect where anomalous eosinophils are characterized by nuclear hypersegmentation, hypogranulation, and negative peroxidase and phospholipid staining.

Similarity:
Belongs to the peroxidase family. XPO subfamily.

Database links:
UniProtKB/Swiss-Prot: P11678.2

important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.