1. Human CD117 ELISA KIT Intended use
The Diaclone Human CD117 ELISA is to be used for the in-vitro quantitative determination of Stem Cell Factor receptor molecule (CD117) in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant CD117.This kit has been configured for research use only.
2. Human CD117 ELISA KIT Principle of the method
The CD117 Kit is a solid phase sandwich Enzyme linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for CD117 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD117 concentrations and unknowns are pipetted into these wells.During thefirst incubation, the CD117 antigen and a biotinylated monoclonal antibody specific for CD117 are simultaneously incubated.After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD117 present in the samples.
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The Diaclone Human CD117 ELISA is to be used for the in-vitro quantitative determination of Stem Cell Factor receptor molecule (CD117) in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant CD117.This kit has been configured for research use only.
2. Human CD117 ELISA KIT Principle of the method
The CD117 Kit is a solid phase sandwich Enzyme linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for CD117 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD117 concentrations and unknowns are pipetted into these wells.During thefirst incubation, the CD117 antigen and a biotinylated monoclonal antibody specific for CD117 are simultaneously incubated.After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD117 present in the samples.
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