1. Human CD14 ELISA Kit Intended use
The Diaclone Human CD14 ELISA Kit is to be used for the in-vitro quantitative determination of CD14 inserum, plasma, or cell lysates. The assay will recognize both natural and recombinant CD14.
This kit has been configured for research use only.
2. Human CD14 ELISA Kit Principle of the method
The CD14 Kit is a solid phase sandwich Enzyme linked-Immuno- Sorbent Assay (ELISA). A monoclonalantibody specific for CD14 has been coated onto the wells of the microtiter strips provided. Samples,including standards of known CD14 concentrations and unknowns are pipetted into these wells.During the first incubation, the CD14 antigen, standards and zeros are bound this is followed by a separate incubation where the biotinylated monoclonal antibody specific for CD14 is added.After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD14 present in the samples.
公司网址：www.craymeibio.com
The Diaclone Human CD14 ELISA Kit is to be used for the in-vitro quantitative determination of CD14 inserum, plasma, or cell lysates. The assay will recognize both natural and recombinant CD14.
This kit has been configured for research use only.
2. Human CD14 ELISA Kit Principle of the method
The CD14 Kit is a solid phase sandwich Enzyme linked-Immuno- Sorbent Assay (ELISA). A monoclonalantibody specific for CD14 has been coated onto the wells of the microtiter strips provided. Samples,including standards of known CD14 concentrations and unknowns are pipetted into these wells.During the first incubation, the CD14 antigen, standards and zeros are bound this is followed by a separate incubation where the biotinylated monoclonal antibody specific for CD14 is added.After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD14 present in the samples.
公司网址：www.craymeibio.com
