DHX螺旋酶抗体实验步骤

中文名称    DHX螺旋酶抗体    
别    名    Ddx32; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 32; DEAD/H box 32; DEAD/H helicase like protein 1; DEAH (Asp-Glu-Ala-His) box polypeptide 32; DEAH box protein 32; DHLP1; DHX32; Helicase DDX32; HuDDX32; Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32; DHX32_HUMAN.    
规   格    0.2ml      
研究领域    肿瘤  信号转导  表观遗传学      
抗体来源    Rabbit    
克隆类型    Polyclonal    
交叉反应    Human, Mouse, Rat, Dog, Pig, Cow, Rabbit,     
产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 DHX螺旋酶抗体（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    
分 子 量    84kDa    
细胞定位    细胞核 细胞浆     
性    状    Lyophilized or Liquid    
浓    度    1mg/1ml    
免 疫 原    KLH conjugated synthetic peptide derived from human DHX32    
亚    型    IgG    
纯化方法    affinity purified by Protein A    
储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    
保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

DHX螺旋酶抗体产品介绍    background:
DEAD-box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure and ribosome/spliceosome assembly. based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX32 is a 743 amino acid nuclear protein that localizes to the mitochondria and is a member of the DEAD box helicase family. Expressed in various tissues, DDX32 is up-regulated by ionomycin in T lymphocytes and down-regulated in acute lymphoblastic leukemia. Considered a novel RNA helicase, DDX32 may play an important role in the development of colorectal cancer and may be involved in regulating T-cell response to certain apoptotic stimuli.

Function:
DHX32 is a member of the family of the DEAD box proteins, a group of proteins that present the conserved motif Asp-Glu-Ala-Asp (DEAD). They have a role in RNA translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly.

Subcellular Location:
Nucleus. Mitochondrion.

Tissue Specificity:
Expressed in lymphoid tissues (at protein level). Expressed in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung, lymphoid tissues and blood leukocytes.

Similarity:
Belongs to the DEAD box helicase family. DEAH subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.

Database links:
UniProtKB/Swiss-Prot: Q7L7V1.1

important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.