脱氧尿苷三磷酸酶DUT抗体操作步骤

中文名称    脱氧尿苷三磷酸酶DUT抗体
英文名称    DUT        
别    名    DUT-N; mitochondrial; Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isofor; Deoxyuridine 5''-triphosphate nucleotidohydrolase; Deoxyuridine triphosphatase; dut; DUT_HUMAN; dUTP nucleotidohydrolase; dUTP pyrophosphatase; dUTPase; FLJ20622.    
规    格    0.1ml  0.2ml      
研究领域    细胞生物  染色质和核信号      
抗体来源    Rabbit    
克隆类型    Polyclonal    
交叉反应    Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,     
产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 脱氧尿苷三磷酸酶DUT抗体（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    
分 子 量    19kDa    
细胞定位    细胞核 细胞浆     
性    状    Lyophilized or Liquid    
浓    度    1mg/1ml    
免 疫 原    KLH conjugated synthetic peptide derived from human DUT (191-237aa)    
亚    型    IgG    
纯化方法    affinity purified by Protein A    
储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    
保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

脱氧尿苷三磷酸酶DUT抗体产品介绍    background:
This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

Function:
This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

Subunit:
Homotrimer.
Subcellular Location:
Isoform 2: Nucleus.
Isoform 3: Mitochondrion.

Tissue Specificity:
Found in a variety of tissues. Isoform 3 expression is constitutive, while isoform 2 expression correlates with the onset of DNA replication (at protein level). Isoform 2 degradation coincides with the cessation of nuclear DNA replication (at protein level).

Post-translational modifications:
Nuclear isoform 2 is phosphorylated in vivo on Ser-11, a reaction that can be catalyzed in vitro by CDC2. Phosphorylation in mature T-cells occurs in a cell cycle-dependent manner. Isoform 3 is not phosphorylated.
The initiator methionine is cleaved in isoform 2.
Similarity:
Belongs to the dUTPase family.