细胞命运决定因子DACH1抗体产品应用_应用方法_资料

中文名称    细胞命运决定因子DACH1抗体
英文名称    DACH1
别    名    DACH; Dach1; DACH1_HUMAN; dachshund homolog 1 (Drosophila); Dachshund homolog 1; Dachsund homolog 1.
规    格    0.2ml
研究领域    肿瘤细胞生物免疫学信号转导转录调节因子
抗体来源    Rabbit
克隆类型    Polyclonal
交叉反应    Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse,
产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 细胞命运决定因子DACH1抗体（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分子量    83kDa
细胞定位    细胞核
性    状    Lyophilized or Liquid
浓    度    1mg/1ml
免疫原    KLH conjugated synthetic peptide derived from human DACH1
亚    型    IgG
纯化方法    affinity purified by Protein A
储存液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

细胞命运决定因子DACH1抗体产品介绍    background:

DACH1 is similar to the Drosophila melanogaster dachshund gene, which encodes a nuclear factor essential for determining cell fates in the eye, leg, and nervous system of the fly. It is a member of the Ski gene family and is thought to be involved in eye development. Four alternatively spliced transcripts encoding different isoforms have been described for this gene. (from Entrez gene). DACH1 has also been found to inhibit oncogene-mediated breast tumor growth via downregulation of cyclin D1. expression of DACH1 has been found to inversely correlate with metastasis.

Function:
Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator of SIX1, SIX6 and probably SIX5. Corepression of precursor cell proliferation in myoblasts by SIX1 is switched to coactivation through recruitment of EYA3 to the SIX1-DACH1 complex. Transcriptional activation seems also to involve association of CREBBP. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter (By similarity). Inhibits TGF-beta signaling through interaction with SMAD4 and NCOR1. Binds to chromatin DNA via its DACHbox-N domain (By similarity).

Subunit:
Interacts with SIX1, SIX6 and EYA3. Interacts with NCOR1 and HDAC3 through its N-terminus. Interacts with SIN3A through its C-terminus. Interacts with SMAD3 and SMAD4.

Subcellular Location:
Nucleus.

Tissue Specificity:
Widely expressed. Isoform 2 is found in brain, heart, kidney, liver, leukocytes and spleen. Isoform 3 is found in liver and heart. Isoform 4 is found in spleen.

Similarity:
Belongs to the DACH/dachshund family.

Database links:

UniProtKB/Swiss-Prot: Q9UI36.2

important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.