三磷酸腺苷依赖解旋酶DDX23抗体使用说明

英文名称    DDX23    
中文名称    三磷酸腺苷依赖解旋酶DDX23抗体    
别    名    100 kDa U5 snRNP-specific protein; DEAD box protein 23; EC 3.6.1.-; PRP28 homolog; U5-100kD; DDX23_HUMAN.    
规    格    0.1ml  0.2ml      
研究领域    细胞生物  细胞分化  表观遗传学      
抗体来源    Rabbit    
克隆类型    Polyclonal    
交叉反应    Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,     
产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 三磷酸腺苷依赖解旋酶DDX23抗体（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    
分 子 量    96kDa    
细胞定位    细胞核     
性    状    Lyophilized or Liquid    
浓    度    1mg/1ml    
免 疫 原    KLH conjugated synthetic peptide derived from human DDX23    
亚    型    IgG    
纯化方法    affinity purified by Protein A    
储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    
保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

三磷酸腺苷依赖解旋酶DDX23抗体产品介绍    background:
DDX23 encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the U5 snRNP complex; it may facilitate conformational changes in the spliceosome during nuclear pre-mRNA splicing. An alternatively spliced transcript variant has been found for this gene, but its biological validity has not been determined.

Function:
Involved in pre-mRNA splicing and its phosphorylated form (by SRPK2) is required for spliceosomal B complex formation.

Subunit:
The phosphorylated form (by SRPK2) associates wth tri-snRNP (U4/U6-U5 tri-small nuclear ribonucleoproteins). Identified in the spliceosome C complex. Interacts with ERBB4.

Subcellular Location:
Nuclear

Post-translational modifications:
In vitro phosphorylated by CLK1 and U1 snRNP-associated protein kinase. Phosphorylated by SRPK2 and this phosphorylation is required for its association with the tri-snRNP (U4/U6-U5 tri-small nuclear ribonucleoproteins) and subsequent spliceosomal B complex formation. [SIMILARITY] Belongs to the DEAD box helicase family. DDX23/PRP28 subfamily.

Similarity:
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.