Precautions
1. To inspect the validity of experiment operation and the appropriateness of sample dilution
proportion, pilot experiment using standards and a small number of samples is
recommended.
2. After opening and before using, keep plate dry.
3. Before using the Kit, spin tubes and bring down all compo
4. Storage TMB reagents avoid light.
5. Washing process is very im
6. Duplicate well assay is recommended for both standard and sample testing.
7. Don’t let Micro plate dry at the assay, for dry plate will inactivate active compo
plate.
8. Don’t reuse tips and tubes to avoid cross contamination.
9. Avoid using the reagents from different batches together.
Material Required but Not Supplied
1. Microplate reader (wavelength: 450nm)
2. 37°C incubator
3. Automated plate washer
4. Precision single and multi-channel pipette and disposable tips
5. Clean tubes and Eppendorf tubes
6. Deio
