使用目的:本试剂盒用于测定进口/国产人血清、血浆及相关液体样本巨噬细胞移动因子(MIF)含量。
The performance of kit:
1 sensitivity: minimum detection concentration is less than 1 standard. LiMIFarity of dilution. Sample liMIFar regression and the expected concentration correlation coefficient R value is 0.990.
2: no specific reaction with other cytokiMIFs.
3 repeaMIFility: plate, plate between the coefficients of variation were less than 10%.
本试剂仅供研究使用 标本:血清或血浆
注:本产品仅供科研使用!
试验原理:
MIF试剂盒是固相夹心法酶联免疫吸附实验(ELISA).已知MIF浓度的标准品、未知浓度的样品加入微孔酶标板内进行检测。先将MIF和生物素标记的抗体同时温育。洗涤后,加入亲和素标记过的HRP。再经过温育和洗涤,去除未结合的酶结合物,然后加入底物A、B,和酶结合物同时作用。产生颜色。颜色的深浅和样品中MIF的浓度呈比例关系。
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