使用目的:本试剂盒用于测定进口/国产大鼠血清、血浆及相关液体样本巨噬细胞炎症蛋白1β(MIP-1β/CCL4)含量。
本试剂仅供研究使用 标本:血清或血浆
注:本产品仅供科研使用!
The performance of kit:
1 sensitivity: minimum detection concentration is less than 1 standard. LiMIP-1β/CCL4arity of dilution. Sample liMIP-1β/CCL4ar regression and the expected concentration correlation coefficient R value is 0.990.
2: no specific reaction with other cytokiMIP-1β/CCL4s.
3 repeaMIP-1β/CCL4ility: plate, plate between the coefficients of variation were less than 10%.
试验原理:
MIP-1β/CCL4试剂盒是固相夹心法酶联免疫吸附实验(ELISA).已知MIP-1β/CCL4浓度的标准品、未知浓度的样品加入微孔酶标板内进行检测。先将MIP-1β/CCL4和生物素标记的抗体同时温育。洗涤后,加入亲和素标记过的HRP。再经过温育和洗涤,去除未结合的酶结合物,然后加入底物A、B,和酶结合物同时作用。产生颜色。颜色的深浅和样品中MIP-1β/CCL4的浓度呈比例关系。
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