Crosslinkers 功能化蛋白交联剂

Protein Crosslinkers 功能化蛋白交联剂,mPEG-NH2，mPEG-DSPE，mPEG-SC，BOC-NH-PEG-NHS，Biotin-PEG-NH2，mPEG-Hydrazide，OH-PEG-NH2，NH2-PEG-NH2，8arm-PEG-SAZ，Boc-NH-PEG2-NH2，COOH-PEG-COOH

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Protein Crosslinkers 功能化蛋白交联剂

蛋白质交联剂是一类小分子化合物，具有2个或者更多的针对特殊基团（-NH2、-COOH、-HS等）的反应性末端，可以和2个或者更多的分子分别偶联，从而使这些分子结合在一起，形成共轭蛋白。

Homo Crosslinkers 同端双功能交联剂 Homobifunctional Crosslinkers 
Protein crosslinking agents possess reactive moieties specific to various functional groups (sulfhydryls, amines, carbohydrates, etc.) on proteins, peptides, or other molecular complexes.
BS3 Crosslinker  BS3 crosslinker (Bis[sulfosuccinimidyl] suberate; BS3) is a water soluble homobifunctional protein crosslinker.
DSS Crosslinker  DSS crosslinker (Disuccinimidyl suberate) is a homobifunctional crosslinker that is cell membrane permeable.
BS2G Crosslinker  BS2G crosslinker (Bis[Sulfosuccinimidyl] glutarate) is a water soluble homobifunctional protein crosslinker.
DSG Crosslinker CAS#: 79642-50-5  DSG crosslinker (Disuccinimidyl glutarate) is a homobifunctional crosslinking reagent that is membrane permeable.
DSP Crosslinker  Dithiobis(succinimidyl propionate) (DSP) is a homobifunctional crosslinker that is cell membrane permeable.
DTSSP Crosslinker  DTSSP crosslinker (3,3′-Dithiobis[sulfosuccinimidylpropionate]) is a homobifunctional protein crosslinker that is cell membrane impermeable.
Sulfo-EGS Crosslinker  Sulfo-EGS crosslinker, Ethylene glycolbis(sulfosuccinimidylsuccinate), is a water soluble homobifunctional crosslinker.
EGS Crosslinker,CAS#: 70539-42-3  EGS crosslinker, Ethylene glycolbis(succinimidylsuccinate), is a water insoluble homobifunctional crosslinker.
DST Crosslinker  DST crosslinker (Disuccinimidyl tartrate) is a homobifunctional, cleavable crosslinking reagent by oxidizing reagents.
DSSeb,CAS # 23024-29-5  DSSeb (Disuccinimidyl sebacateis membrane permeable. DSSeb crosslinks via amine-reactive NHS esters at both ends of a 10-atom (14.1 Å ) spacer arm and must dissolved in an organic sol-vent, such as DMSO or DMF, then added to an aqueous crosslinking reaction

Hetero Crosslinkers 异端双功能交联剂 Heterobifunctional Crosslinkers
Protein crosslinking agents possess reactive moieties specific to various functional groups (sulfhydryls, amines, carbohydrates, etc.) on proteins, peptides, or other molecular complexes.
SMCC Crosslinker  SMCC crosslinker (Succinimidyl-4-[N-maleimidomethyl]cyclohexane-1-carboxylate) is a heterobifunctional protein crosslinker with a maleimide group that is sulfhydryl (thiol; -SH) reactive and a NHS ester group that is amine reactive.
Sulfo-SMCC Crosslinker  Sulfo-SMCC crosslinker (Sulfosuccinimidyl-4-[N-maleimidomethyl]cyclohexane-1-carboxylate) is a water soluble, heterobifunctional crosslinker.
Sulfo-SANPAH Crosslinker  Sulfo-SANPAH crosslinker (N-Sulfosuccinimidyl-6-[4'-azido-2'-nitrophenylamino] hexanoate) is a water soluble, heterobifunctional protein crosslinker with a spacer arm length of 18.2 Å.
SMPH Crosslinker  SMPH crosslinker (Succinimidyl-6-[ß-maleimidopropionamido]hexanoate) is a water insoluble, heterobifunctional protein crosslinker with a non-cleavable spacer arm length of 14.3 angstroms.
PDPH Crosslinker  PDPH is a cleavable, heterobifunctional protein crosslinking reagent.
MBS Crosslinker CAS#: 58626-38-3  MBS crosslinker is a non-cleavable, water insoluble, heterobifunctional protein crosslinking reagent that is commonly used to crosslink haptens to carrier proteins and enzymes to antibody.
Sulfo-MBS Crosslinker  Sulfo-MBS crosslinker (m-Maleimidobenzoyl-N-hydroxysulfosuccinimide ester) is a non-cleavable, water soluble, heterobifunctional protein crosslinking reagent that is commonly used to crosslink haptens to carrier proteins and enzymes to antibody.
SPDP Crosslinker  SPDP crosslinker(68181-17-9) is a cleavable, water insoluble, amino and thiol (sulfhydryl) reactive heterobifunctional protein crosslinker.
LC-SPDP Crosslinker  LC-SPDP crosslinker is a cleavable, water insoluble, amino and thiol (sulfhydryl) reactive heterobifunctional protein crosslinker.
Sulfo-LC-SPDP Crosslinker  Sulfo-LC-SPDP crosslinking reagent is a cleavable, water soluble, amino and thiol (sulfhydryl) reactive heterobifunctional protein crosslinker.
SIA Crosslinker  N-Succinimidyl iodoacetate (SIA) is a non-cleavable, heterobifunctional protein crosslinker.
SBA Crosslinker  SBA crosslinker (N-Succinimidyl bromoacetate) is a sulfhydryl (thiol) and amino reactive heterobifunctional protein crosslinking reagent.
SMPB Crosslinker  SMPB crosslinker (N-Succinimidyl 4-[4-maleimidophenyl]butyrate) is a heterobifunctional protein crosslinking reagent that is reactive toward sulfhydryl and amino groups.
BMPS Crosslinker  BMPS is a non-cleavable, water insoluble, heterobifunctional protein crosslinker that is commonly used to crosslink haptens to carrier proteins and enzymes to antibody.
EMCS Crosslinker,CAS#: 55750-63-5  N-(e-Maleimidocaproyloxy)succinimide ester (EMCS) is a non-cleavable, water insoluble, heterobifunctional crosslinker that is commonly used to crosslink haptens to carrier proteins and enzyme to antibody.
GMBS Crosslinker CAS#: 80307-12-6  GMBS is a non-cleavable heterobifunctional protein crosslinker.
ANB-NOS Crosslinker  ANB-NOS crosslinker (N-5-Azido-2-nitrobenzoyloxysuccinimide) is a heterobifunctional protein crosslinker with a spacer arm length of 7.7 angstroms.
LC-SMCC Crosslinker  LC SMCC is a non cleavable and membrane permeable crosslinker.
BMPH (N-β-maleimidopropionic acid hydrazide) CAS#: 359436-61-6  BMPH is a short, maleimide-and-hydrazide crosslinker for conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).

Biotin Crosslinkers 生物素交联试剂 Biotinylation Reagents
Protein crosslinking agents possess reactive moieties specific to various functional groups (sulfhydryls, amines, carbohydrates, etc.) on proteins, peptides, or other molecular complexes.
Sulfo-NHS-Biotin  Sulfo-NHS-Biotin (Sulfosuccinimidyl biotin) is a water soluble biotinylation reagent.
NHS-Biotin  NHS-Biotin (Succinimidyl Biotin; Succinimidobiotin) is a primary amine reactive biotinylation reagent.
Biotin-PFP 五氟苯酚生物素酯 CAS#120550-35-8  Biotin-PFP 五氟苯酚生物素酯 CAS#120550-35-8
Sulfo-NHS-LC-Biotin  Sulfo-NHS-LC-Biotin, Sulfosuccinimidyl 6-(biotinamido) Hexanoate, is a long chain water soluble biotinylation reagent that is reactive toward primary amine groups.
NHS-LC-Biotin  Succinimidyl 6-(biotinamido)hexanoate (NHS-LC-Biotin) is an amino-reactive biotinylation reagent with a long spacer arm.
Sulfo-NHS-SS-Biotin  Sulfosuccinimidyl-2-(biotinamido)-ethyl-1,3'-dithiopropionate (Sulfo-NHS-SS-Biotin) is a cleavable, water-soluble, amino-reactive biotinylation reagent.
N-琥珀酰亚氨基6-生物素氨己酸 CAS#:72040-63-2  N-琥珀酰亚氨基6-生物素氨己酸 CAS#:72040-63-2 Biotinamidohexanoic acid N-hydroxysuccinimide ester
6-生物素氨基己酸,CAS#:72040-64-3  6-生物素氨基己酸,CAS#:72040-64-3,N-(+)-Biotinyl-6-aminohexanoic acid
6-[生物素酰氨基]己酸磺基琥珀酰亚胺酯 CAS#:127062-22-0  6-[生物素酰氨基]己酸磺基琥珀酰亚胺酯 CAS#:127062-22-0 ,Biotinamidohexanoic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt
NHS-SS-Biotin  NHS-SS-Biotin, Succinimidyl-2-(biotinamido)-ethyl-1,3'-dithiopropionate, is an amine-reactive biotinylation reagent that contains a reducible disulfide.
Biotin Hydrazide  Biotin Hydrazide (Biotin-Hz) is a carbohydrate reactive biotinylation reagent. CAS#：66640-86-6
Biotin-HPDP  N-[6-(Biotinamido)hexyl]-3’-(2’-pyridyldithio)-propionamide (Biotin-HPDP) is a sulfhydryl reactive biotinylation reagent with a long chain, cleavable spacer arm.
Iodoacetyl-LC-Biotin  Iodoacetyl-LC-Biotin (N-Iodoacetyl-N'-Biotinylhexylenediamine or N-Biotinyl-N'-(iodoacetyl)-1,6-hexanediamine) reacts with sulfhydryl groups to form stable thioether linkages.
D-Biotin  D-Biotin, often called Vitamin H, D-(+)-Biotin, or simply Biotin, is water soluble. D-Biotin is often used in conjunction with Streptavidin or Immobilized Streptavidin.
Biotin-ONP 生物素-4-硝基苯酯 CAS#33755-53-2  Biotin-ONP (+)-生物素-4-硝基苯酯 cas#33755-53-2
Biotin-PEG3-CH2CH2NH2  3个重复单元的小分子PEG, 一端连生物素，一段连氨基，分子量是418.55，CAS：359860-27-8
Biotin-PEG3-CH2CH2COOH  3个重复单元的小分子PEG, 一端连生物素，一段连羧基，分子量是447.54，CAS：252881-76-8
Biotin-PEG3-Hydrazide  Biotinylation reagent with Hydrazide, and a3 PEG spacer.
Biotin-PEG4-CH2CH2N3  Biotinylation reagent with Azide, and a 3 PEG spacer
Biotin-NH-CH2CH2-S-S-pyr  N/A
Biotin-PEG3-NH-Mal  Biotinylation reagent with NH-Mal, and a 3 PEG spacer
Biotin-LC  Biotinyl-6-aminohexanoic acid (Biotin-X) may be used in the biosynthesis of biotinylated oligosaccharides. Biotinyl-6-aminohexanoic acid is frequently use as a derivative N-hydroxysuccinimide ester.
Biotin-LC-LC  A biotinylated cross-linking reagent
Biotin-SLC-NHS Ester  N-Hydroxysulfosuccinimide (NHS) esters of biotin are the most popular type of biotinylation reagents and NHS-activated biotins react efficiently with primary amino groups (-NH2) in alkaline buffers to form stable amide bonds. Extended chain increases avidin or streptavidinbinding and reduces potential steric hindrance of biotin and conjugated molecule.
Biotin-ONP  A biotinylated cross-linking reagent
Biotin-LC-Hydrazide  
Biotin-LC-LC-NHS Ester  NHS-LC-LC-Biotin is a doubly long-chain, NHS-ester activated biotinylation reagent for labeling primary amines (e.g., protein lysines), whose membrane permeability enables it to be used for intracellular labeling.
Sulfo-NHS-LC-LC-Biotin  Sulfo-NHS-LC-LC-Biotin is a long-chain biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.
PFP-Biotin  Biotinylation reagent with a pentafluorophenyl ester group. More reactive than the NHS ester group, targeting both primary and secondary amines. Suitable for biotin labeling of both proteins and amino acids.
Biotin–cadaverine  Biotin and biotin derivatives bind with high affinity to both avidins and streptavidins.
Fmoc-D-Lys(Biotin)-OH  A biotinylated cross-linking reagent

Other Crosslinkers 其他交联剂产品 Other Modifications
Protein crosslinking agents possess reactive moieties specific to various functional groups (sulfhydryls, amines, carbohydrates, etc.) on proteins, peptides, or other molecular complexes.
SATA Protein Modifier  N-Succinimidyl-S-Acetyl-Thioacetate (SATA) is used to convert primary amine groups into protected sulfhydryl (thiol; -SH) groups.
(KMUS), CAS#:87981-04-2  11-马来酰亚胺十一烷酸 N-琥珀酰亚胺酯(KMUS),CAS#:87981-04-2
Sulfo-NHS-Acetate  Sulfo-NHS-Acetate acylating agent (Sulfosuccinimidyl Acetate) is a water soluble.
Traut's Reagent  Traut's Reagent (2-Iminothiolane HCl) is commonly used to convert primary amines into sulfhydryl groups in a one-step process is called thiolation.
3-马来酰亚胺基丙酸(MPA), CAS#:7423-55-4  3-马来酰亚胺基丙酸(MPA), CAS#:7423-55-4
4-马来酰亚胺丁酸(MBA) CAS#:57078-98-5  4-马来酰亚胺丁酸(MBA) CAS#:57078-98-5
11-马来酰亚胺十一烷酸(KMUA),CAS#57079-01-3  11-马来酰亚胺十一烷酸(KMUA),CAS#57079-01-3
HPG  HPG protein modification reagent (p-Hydroxyphenylglyoxal) reacts with arginine residues to give a large increase in absorbance at approximately 340 nm.
Iodoacetic Acid  Iodoacetic Acid is ≥ 99.0% pure. Iodoacetic acid is used for the modification and alkylation of sulfhydryls (-SH) in cysteine residues found in proteins and peptides.
2-马来酰亚胺基乙酸(MAA),CAS#25021-08-3  2-马来酰亚胺基乙酸(MAA),CAS#25021-08-3
Sulfo-EMCS,CAS#:215312-86-0  Sulfo-EMCS,CAS#:215312-86-0
CAS55750-61-3,马来酰亚胺基乙酸琥珀酰亚胺酯(AMAS)  CAS55750-61-3,马来酰亚胺基乙酸琥珀酰亚胺酯(AMAS)
双马来酰亚胺基乙烷,CAS#:5132-30-9  双马来酰亚胺基乙烷,CAS#:5132-30-9
2-(2-(2-氨基乙氧基)乙氧基)乙基氨基甲酸叔丁酯,CAS#:153086-78-3  2-(2-(2-氨基乙氧基)乙氧基)乙基氨基甲酸叔丁酯,CAS#:153086-78-3
N,N,N',N'-四甲基对苯二胺,CAS#:100-22-1  N,N,N',N'-四甲基对苯二胺,CAS#:100-22-1
3,3'-二硫代双(磺酸琥珀酰亚氨基丙酸酯) CAS#:81069-02-5  3,3'-二硫代双(磺酸琥珀酰亚氨基丙酸酯) CAS#:81069-02-5
BMPA Modifier  BMPA Modifier (N-ß-Maleimidopropionic acid) can be used as a protein crosslinker or sulfhydryl (-SH) to carboxylic acid (-COOH) modification reagent.
EDC-HCl (EDAC-HCl)  EDC-HCl (EDAC-HCl) zero-length crosslinker from ProteoChem is UltraPure, and is used to conjugate carboxyl functional groups to primary amines as found in peptides and proteins.
NHS  N-hydroxysuccinimide (NHS) prolongs the lifetime of amino-reactive moieties formed at carboxylic acids and increases the efficiency.
Sulfo-NHS  N-hydroxysulfosuccinimide (Sulfo-NHS) is used in conjunction with EDC-HCl (EDAC-HCl) to convert carboxyl groups to Sulfo-NHS esters which react amines.
DCC  N,N'-Dicyclohexylcarbodiimide (DCC) is a zero length coupling reagent.
CDI  N,N'-Carbodiimide (CDI) is a zero length crosslinking reagent used to couple primary amines to carboxyllic acid functional groups.
IPTG, CAS#:367-93-1  异丙基1-硫代-β-D-半乳糖吡喃糖苷（IPTG）是β–半乳糖苷酶的活性诱导物质，常用于蓝白斑筛选及IPTG诱导的细菌内的蛋白表达等。当pUC系列的载体DNA（或其他带有lacZ 基因载体DNA）以lacZ 缺失细胞为宿主进行转化时、或用M13噬菌体的载体DNA进行转染时，如果在平板培养基中加入X–Gal和IPTG，由于β–半乳糖苷酶的α–互补性，可以根据是否呈现白色菌落（或噬菌斑）而方便地挑选出基因重组体。此外，它还可以作为具有lac或tac等启动子的表达载体的表达诱导物使用。
PNPP,CAS#:4264-83-9  4-硝基苯基磷酸二钠盐 CAS#:4264-83-9
6-马来酰亚胺基己酸 CAS#:55750-53-3  6-Maleimidohexanoic acid may be used as a spacer in the construction of drug and other types of bioconjugates. 6-Maleimidohexanoic acid is used with N-hydroxysuccinimide ester as a bifunctional cross-linking reagent.Probe for thiol groups (SH-groups) in membrane proteins.
6-(叔丁氧羰基-氨基)己酸 CAS, #51513-80-5  6-(叔丁氧羰基-氨基)己酸 CAS, #51513-80-5
TMPD,CAS#: 637-01-4  N,N,N',N'-四甲基对苯二胺二盐酸盐
碘乙酸,CAS#: 39028-27-8  碘乙酸 N-羟基琥珀酰亚胺酯
TSAT (tris-(succinimidyl)aminotriacetate)  TSAT is tris-(succinimidyl) aminotriacetate, a novel trifunctional crosslinker that contains three short spacers arms linked to a central nitrogen atom, each ending in an amine-reactive NHS-ester group.
DST (disuccinimidyl tartrate)  This product is sensitive to moisture. The vial is packaged in a resealable bag with a desiccant to reduce exposure to moisture. After cold storage, equilibrate the vial to room temperature before opening to reduce condensation inside the vial. Make fresh solutions. Storage of stock solutions is not recommended. After use, return the vial to the resealable bag. Close the bag and store the product at the recommended temperature.
EMCH (N-ε-maleimidocaproic acid hydrazide) CAS#: 151038-94-7  EMCH is a mid-length, maleimide-and-hydrazide crosslinker for conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).
KMUH (N-κ-maleimidoundecanoic acid hydrazide) CAS#: 1593661-78-9  马来酰亚胺基十一烷酰肼三氟乙酸盐
BMB (1,4-bismaleimidobutane) CAS#: 28537-70-4  BMB is a mid-length, maleimide crosslinker for covalent, irreversible conjugation between sulfhydryl groups (e.g., protein or peptide cysteines).
BMH (bismaleimidohexane) CAS#:4856-87-5  BMH is a long-arm, maleimide crosslinker for covalent, irreversible conjugation between sulfhydryl groups (e.g., protein or peptide cysteines).
TMEA ,CAS#: 139112-38-2  TMEA is a trifunctional crosslinker containing three reactive maleimide groups to enable three-way conjugation and polymerization of sulfhydryl-containing molecules.
DTME CAS#:71865-37-7  This product is sensitive to moisture. The vial is packaged in a resealable bag with a desiccant to reduce exposure to moisture. After cold storage, equilibrate the vial to room temperature before opening to reduce condensation inside the vial. Make fresh solutions. Storage of stock solutions is not recommended. After use, return the vial to the resealable bag. Close the bag and store the product at the recommended temperature. General description DTME is a homobifunctional, maleimide crosslin
SATP , CAS#: 84271-78-3  Thermo Scientific Pierce SATP is a long-chain (4.1 angstrom spacer arm) reagent for covalent modification of primary amines and addition of a protected yet exposable sulfhydryl group, enabling heterobifunctional crosslinking strategies.
AEDP  AEDP is an amine-and-carboxyl compound with an internal disulfide bond. AEDP enables construction, extension and modification of several functional groups on proteins and surfaces.
N-丙烯酰氧琥珀酰亚胺, CAS#: 38862-24-7  N-丙烯酰氧琥珀酰亚胺, CAS#: 38862-24-7

Zero-Length Crosslinkers 零长度交联剂
Sulfo-NHS

Protein Crosslinkers 功能化蛋白交联剂

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Protein Crosslinkers 功能化蛋白交联剂

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聚乙二醇修饰化学和PEG修饰剂选择指南
移液器的日常清洗和维护保养
样品瓶和隔垫的选择指南

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