BD-EL-H1548 人抗肝特异性脂蛋白(LSP)酶联免疫吸附测定试剂盒

Take a kit from 2-8 centigrade environment, balance it for at least 30minutes in room temperature before opening. ELISA plates coated if has not use up after opened

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.  Take a kit from 2-8 centigrade environment, balance it for at least 30minutes in room temperature before opening. ELISA plates coated if has not use up after opened, the plate should be stored in sealed bag.

2. Operate strictly in accordance with instructions, the microtiter plate reader must be as the criterion of the test result determination.

3. To avoid cross contamination, do not repeatedly use the tip and seal plate membrane in the hands.

4. Other unused agents need to be well-packed or covered. Do not mix different batches of reagents. Use before the expiration date..

5. Substrate B is sensitive to light, avoid long time exposure to light.             

6. All samples, washing buffer and each kind of reject should be processed by biological waste treatment. Stop Solution is 2M sulfuric acid, must be cautious when using.

7. Use a pipette for  each step of sampling, and often calibrate its accuracy to avoid the experimental error. Each sampling time should be best controlled in five minutes, if there are a large number of sample, a Volley is recommended for use.

8. Make a standard curve for each test. If the content of testing material in the sample is too high (sample OD value is greater than that of the highest concentration in standard hole ), first dilute sample dilution with a certain times (n-time) and then test, please multiply by the diluted times when calculating (× n).

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