产品类型:ELISA Kit- 产品名称:
大鼠FK506结合蛋白12-雷帕霉素相关蛋白1(FRAP1) ELISA kit
- 英文名称:
Rat FK506 binding protein 12-rapamycin associated protein 1(FRAP1) ELISA kit
- 货号:
CSB-EL008968RA
- 规格:
96T
- 种属:
Rat
- 其他种属:
- 待测物名称:
FK506 binding protein 12-rapamycin associated protein 1(FRAP1)
- 缩写:
FRAP1
- 检测范围:
0.156 ng/ml-10 ng/ml
- 灵敏度:
0.039 ng/ml
- 反应时间:
1-5h
- 原理:
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FRAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ......+
- 特异性:
This assay has high sensitivity and excellent specificity for detection of Rat FRAP1. No significant cross-reactivity or interference between Rat FRAP1 and analogues was observed.
- 度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess. - 样本搜集及储存:
Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediat......+
- 检测步骤:
Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1.......+
- 结果计算:
Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract ......+









