型号:JKY/K-PHYT 植酸/总磷量检测试剂盒 库号：M316051

植酸/总磷量检测试剂盒 库号：M316051

东方化玻（北京）科技有限公司成立于 2014 年，是实验设备、平台综合解决方案提供商。公司为国内企事业单位 提供高品质仪器仪表，实验设备以及整体解决方案。 公司与国内外多家世界生产商已建立长期合作关系。公司经营产品广泛，灵活满足不同事业单位和企业客户的差异化需求 以及快速创新的追求，产品故障率低，维护成本小，性能优异，售后服务完善。公司产品在行业内均拥有持续的应用历史并 经过市场实际应用检验。 坚持以持续技术创新为客户不断创造价值为经营理念，公司在产品研发方面投入比例逐年递增，目前自主研发并投入市场 的产品已达十余种，长期以来，公司一直按照标准，搭建实验室平台，构建高品质、高标准、持续创新的发展体系。 坚持稳步发展的管理理念，不断刷新自己在业内的记录。

植酸/总磷量检测试剂盒 库号：M316051
植酸/总磷量检测试剂盒 库号：M316051
植酸/总磷量检测试剂盒 库号：M316051
名称：植酸/总磷量检测试剂盒 
产品货号：K-PHYT 
规格：50 
产地：爱尔兰 
数量：大量 

英文名：Phytic Acid/Total Phosphorus Assay Kit
保存条件：4-6摄氏度
保质期：1年以上



外观四个玻璃小瓶和两个60毫升聚丙烯瓶

比重 不适用
在水中的溶解度易溶
pH值 7.0
气味 无
粉末和液体形式

稳定性 稳定 十年或以上(冷冻机)

成分:
名称 比例
醋酸钠缓冲液60毫升 一小瓶（聚丙烯）
甘氨酸缓冲液 一瓶（聚丙烯）
植酸酶悬浮液 一个七毫升小瓶（玻璃）
碱性磷酸酶悬浮液 一个七毫升小瓶（玻璃）
磷标准溶液25毫升 一小瓶（玻璃）
燕麦粉的控制液 一二毫升小瓶（玻璃）


试剂盒：
试剂盒适合执行50个化验可从
包含完整的检测方法，另加：
瓶1：醋酸钠缓冲液（25毫升，200毫米，pH值5.5）和
叠氮化钠（0.02％瓦特/视频作为防腐剂）。
稳定在4 ° C的“2年
瓶2：植酸酶暂停（1.2毫升，12000单位/毫升）。
稳定在4 ° C的“2年
瓶3：甘氨酸缓冲液（25毫升，400毫米，pH值10.4），加氯化镁
（4毫米），硫酸锌（0.4毫米）和叠氮化钠
（0.02％瓦特/ 5）作为防腐剂。
稳定在4 ° C的“2年
瓶4：暂停碱性磷酸酶（1.2毫升，80单位/毫升）。
稳定在4 ° C的“2年
瓶5：磷标准溶液（24毫升，50微克/毫升）。
和叠氮化钠（0.02％瓦特/视频作为防腐剂）。
稳定在4 ° C的“2年
瓶6：燕麦粉控制粉（5克，磷含量，
见瓶）。
稳定“在室温下5年。

Phytic Acid / Total Phosphorus
A simple method for the measurement of phytic acid / total phosphorus in food and feed samples. This method does not require purification of phytic acid via anion-exchange chromatography making it amenable to high numbers of samples.

Catalogue Number: K-PHYT
Content: 50 assays

Appearance Four glass vials and two 60 mL polypropylene containers.
Specific Gravity Not applicable
Solubility in Water Readily soluble.
pH Value 7.0
Odour none
Form powders and liquids
Stability stable in a freezer for ten or more years
Ingredients Name CAS Proportion
Sodium acetate buffer One 60 ml vial (polypropylene)
Glycine Buffer One container (polypropylene)
Phytase suspension One 7 ml vial (glass)
Alkaline phosphatase suspension One 7 ml vial (glass)
Phosphorus standard solution One 25 ml vial (glass)
Oat flour control One 12 ml vial (glass)


KITS:
Kits suitable for performing 50 assays are available.The
kits contain the full assay method plus:
Bottle 1: Sodium acetate buffer (25 mL, 200 mM, pH 5.5) and
sodium azide (0.02 % w/v) as a preservative.
Stable for > 2 years at 4°C.
Bottle 2: Phytase suspension (1.2 mL, 12,000 U/mL).
Stable for > 2 years at 4°C.
Bottle 3: Glycine buffer (25 mL, 400 mM, pH 10.4), plus MgCl2
(4 mM), ZnSO4 (0.4 mM) and sodium azide
(0.02 % w/v) as a preservative.
Stable for > 2 years at 4°C.
Bottle 4: Alkaline phosphatase suspension (1.2 mL, 80 U/mL).
Stable for > 2 years at 4°C.
Bottle 5: Phosphorus standard solution (24 mL, 50 μg/mL).
and sodium azide (0.02 % w/v) as a preservative.
Stable for > 2 years at 4°C.
Bottle 6: Oat flour control powder (5 g; Phosphorus content,
see bottle).
Stable for > 5 years at room temperature.


INTRODUCTION:
Phytic acid (phytate; myo-inositol 1,2,3,4,5,6, hexakisphosphate) is the
primary source of inositol and storage phosphorus in plant seeds
contributing ~70 % of total phosphorus. The abundance of phytic acid
in cereal grains is a concern in the foods and animal feeds industries
because the phosphorus in this form is unavailable to monogastric
animals due to a lack of endogenous phytases; enzymes specific for
the dephosphorylation of phytic acid. In addition, the strong chelating
characteristic of phytic acid reduces the bioavailability of other
essential dietary nutrients such as minerals (e.g. Ca2+, Zn2+, Mg2+,
Mn2+, Fe2+/3+), proteins and amino acids.1 High phytic acid content
feeds are generally supplemented with inorganic phosphate, however
this causes increased faecal phosphate levels and subsequent
eutrophication of waterways. Alternatively, supplementation with
commercial phytases is becoming increasingly popular and reduces the
requirement for inorganic phosphate supplementation as well as the
associated environmental issues.
Currently, there is no commercially available, simple, quantitative
method for phytic acid and while such measurement is relatively
complex the generally accepted AOAC Method 986.11 has
limitations.2 For each individual analysis the method requires
cumbersome anion-exchange purification and a major inherent
assumption here is that only phytic acid is purified. While this
assumption is viable for non-processed grains for which phytic acid
comprises at least 97 % of total inositol phosphates, it is not viable for
processed foods and feeds which can contain higher levels of some
lower myo-inositol phosphate forms (i.e. IP3, IP4, IP5) that would coelute
with phytic acid and contribute to overestimation of the phytic
acid content.3,4
Given the complexities of the purification and measurement of phytic
acid separate from lower myo-inositol phosphate forms, Megazyme
has developed a simple, quantitative method (K-PHYT) to measure
total “available phosphorus” released from food and feed samples that
is amenable to high numbers of samples and does not require tedious
anion-exchange purification. This method involves acid extraction of
inositol phosphates followed by treatment with a phytase that is
specific for phytic acid (IP6) and the lower myo-inositol phosphate
forms (i.e. IP2, IP3, IP4, IP5). Subsequent treatment with alkaline
phosphatase ensures the release of the final phosphate from myoinositol
phosphate (IP1) which is relatively resistant to the action of
phytase. The total phosphate released is measured using a modified
colorimetric method and given as grams of phosphorus per 100 g of
sample material.5,6
1
Alkaline phosphatase will also release phosphate from
monophosphate esters other than myo-inositol phosphate, however
analyses of a broad spectrum of samples using K-PHYT indicated that
phosphorus released from monophosphate esters other than myoinositol
phosphate is small.


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