小鼠IL-2 ELISpot PLUS试剂盒(ALP)

小鼠IL-2 ELISpot PLUS试剂盒(ALP)种属反应性： Mouse

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中文名称：小鼠IL-2 ELISpot PLUS试剂盒(ALP)
英文名称：Mouse IL-2 ELISpot PLUS kit (ALP)
品牌：Mabtech
规格：2plates
检测方法：Colorimetric
样品类型：Suspension cells
检测类型：Sandwich (qualitative)
实验步骤：Multiple steps standard assay
种属反应性： Mouse小鼠IL-2 ELISpot PLUS试剂盒(ALP)


小鼠IL-2 ELISpot PLUS试剂盒(ALP)产品概述
The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.

Abcam Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

Principal

After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells.
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