2533 p53 (1C12) Mouse mAb (Alexa Fluor 647 Conjugate) #2533

"上海博耀生物销售p53 (1C12) Mouse mAb (Alexa Fluor 647 Conjugate) #2533，CST原装品质，价格优惠，订购p53 (1C12) Mouse mAb

"上海博耀生物销售p53 (1C12) Mouse mAb (Alexa Fluor 647 Conjugate) #2533，CST原装品质，价格优惠，订购p53 (1C12) Mouse mAb (Alexa Fluor 647 Conjugate) #2533，详情请咨询销售人员！
产品详细参数如下>>>>

产品名称：p53 (1C12) Mouse mAb (Alexa Fluor 647 Conjugate) #2533
货号：2533
品牌：CST(cell signaling)
抗体来源：Mouse
克隆类型： Monoclonal
规格：

No.	Size	Price
2533S	100 ul (50 tests)	询价

抗体应用：

Applications	Reactivity	Sensitivity	Isotype
IF-IC F	H M R Mk	Endogenous	Mouse IgG1

Applications Key:  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Mon key
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on sequence homology.
特异性/敏感度： p53 (1C12) Mouse mAb (Alexa Fluor^® 647 Conjugate) detects endogenous levels of total p53 protein.
来源及纯化方法： Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser20 of human p53. The antibody was conjugated to Alexa Fluor^® 647 under optimal conditions with an F/P ratio of 2-6. The Alexa Fluor^® 647 dye is maximally excited by red light (e.g. 633 nm He-Ne laser). Antibody conjugates of the Alexa Fluor^® 647 dye produce bright far-red-fluorescence emission, with a peak at 665 nm.
抗体背景：

The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro (2,3). DNA damage induces phosphorylation of p53 at Ser15 and Ser20 and leads to a reduced interaction between p53 and its negative regulator, the oncoprotein MDM2 (4). MDM2 inhibits p53 accumulation by targeting it for ubiquitination and proteasomal degradation (5,6). p53 can be phosphorylated by ATM, ATR, and DNA-PK at Ser15 and Ser37. Phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and activation of p53 in response to DNA damage (4,7). Chk2 and Chk1 can phosphorylate p53 at Ser20, enhancing its tetramerization, stability, and activity (8,9). p53 is phosphorylated at Ser392 in vivo (10,11) and by CAK in vitro (11). Phosphorylation of p53 at Ser392 is increased in human tumors (12) and has been reported to influence the growth suppressor function, DNA binding, and transcriptional activation of p53 (10,13,14). p53 is phosphorylated at Ser6 and Ser9 by CK1δ and CK1ε both in vitro and in vivo (13,15). Phosphorylation of p53 at Ser46 regulates the ability of p53 to induce apoptosis (16). Acetylation of p53 is mediated by p300 and CBP acetyltransferases. Inhibition of deacetylation suppressing MDM2 from recruiting HDAC1 complex by p19 (ARF) stabilizes p53. Acetylation appears to play a positive role in the accumulation of p53 protein in stress response (17). Following DNA damage, human p53 becomes acetylated at Lys382 (Lys379 in mouse) in vivo to enhance p53-DNA binding (18). Deacetylation of p53 occurs through interaction with the SIRT1 protein, a deacetylase that may be involved in cellular aging and the DNA damage response (19).

关键词：CST，抗体，一抗{byao_back}{byao_back2}

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