5-叔丁酯

人肝癌细胞；BEL-7402 上皮细胞样贴壁生长人肝癌细胞；BEL-7402BEL-7402细胞株是1974年从临床肝癌手术标本中建立的。 细胞群体倍

人肝癌细胞；BEL-7402
上皮细胞样
贴壁生长
人肝癌细胞；BEL-7402BEL-7402细胞株是1974年从临床肝癌手术标本中建立的。 细胞群体倍增时间为20小时。 移植到处理过的wistar大鼠中的能形成肿瘤结节。 细胞形态呈上皮样细胞。 在电子显微镜下亦显示上皮细胞所具有的桥粒和张力原纤维，与临床肝癌细胞相似。 分瓶培养第四天时，其有丝分裂指数约为7%。 BEL-7402细胞的染色体数为不足三倍体，有一个异常的近端着丝点染色体。 间接免疫荧光法测BEL-7402细胞内的AFP为阳性反应。 LDH同工酶谱显示与成年人肝细胞不同，而与人胚肝及临床肝癌相近。
RPMI 1640 (w/o Hepes) 胎牛血清，10%
消化3-5分钟。1:2。3天内可长满。
不详
完全培养基+8%DMSO
X30618 5 x 10^5 cells/vial
X30619 5 x 10^5 cells/vial
X30620 5 x 人肝癌细胞；BEL-740210^5 cells/vial
X30621 5 x 10^5 cells/vial
X30622 5 x 10^5 cells/vial
X30623 5 x 10^5 cells/vial
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X30635 1 x 10^6 cells/vial
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X30662 1 x 10^6 cells/vial
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X30666 5 x 10^5 cells/vial
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X30676 1 x 10^6 cells/vial
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X30680 1 x 10^6 cells/vial
X30681 1 x 10^6 cells/vial
X30682 1 x 10^6 cells/vial
X30683 5 x 10^5 cells/vial
X30684 5 x 人肝癌细胞；BEL-740210^5 cells/vial

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