SF/Z JD0107001-2016 Determination of ethanol in blood Headspace gas chromatography

SF/Z JD0107001-2016 Determination of ethanol in blood Details of headspace gas chromatography:



Haohan Chromatography (Shandong) Applied Technology Development Co., Ltd. has established a method for rapid determination of ethanol content in whole blood by headspace injection capillary column gas chromatography. Method: The sample is equilibrated at 60 ℃ for 15min, and the upper gas is taken for 30 μ L Headspace injection, split ratio is 1, and tert-butanol is used as the internal standard for quantification. Results: Ethanol and tert-butanol could be completely separated under the chromatographic conditions; The linear range of the method is 20-160mg/100mL, R=0.9979, the detection limit is 5mg/100mL, the recovery rate of spiking is 92.5-96.3%, and the RSD is less than 5%. Conclusion: The method is simple, accurate, sensitive and reproducible, and can be used for the determination of ethanol in blood.




HH-ALC2（30m × 0.32mm × one point two μ m) Quartz capillary column or equivalent; Column temperature: constant temperature 40 ° C




SF/Z JD0107001-2016 Determination of ethanol in blood headspace gas chromatography spectrogram: