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价格：电议
所在地：上海
型号：货号CYT-222
更新时间：2012-11-04
浏览次数：877
公司地址：上海市莲花南路1108弄
林小姐(女士)
上海亚培生物科技有限公司，是一家专注于生命科学和生物技术领域的高科技企业，专业提供分子生物学、免疫学、生命科学基础研究以及临床检测等诸多领域的试剂、耗材、仪器等各类产品及生物技术服务。 公司一方面注意跟踪世界范围分子生物学、细胞生物学等生物学科的发展前沿，努力将欧美专业生产厂家的具有国际先进水平的产品推荐给各类研究人员；另一方面也非常重视自主产品研究和开发，推出了一系列具有竞争力的产品和服务。公司客户遍布大学、研究所、医院、卫生防疫、商品检验检疫、制药公司、生物技术公司和食品工业等单位。 公司在重视产品质量的同时，也建立了一套集技术支持、物流、售后服务等多个部门的全方位的服务体系，努力把我们方便、快捷、周到的服务提供给每一个客户。我们的目标是让亚培成为在生命科学和生物技术领域内集生产、研究开发和销售于一体的国内外知名的一流企业每一个亚培人，都心存一个信念：希望通过自身的不懈努力，通过我们优秀的产品、专业的服务来更好的服务用户，为我们每一个用户的成功铺路搭桥，也为亚培的成功铺路搭桥！
| Catalogue Number | CYT-222 | |||||||||
| Synonyms | CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim. | |||||||||
| Introduction | GMCSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. |
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| De |
Granulocyte Macrophage Colony Stimulating Factor Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 125 amino acids and having a molecular mass of 14285.35 Dalton. GM-CSF Mouse is purified by proprietary chromatographic techniques. |
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| Source | Escherichia Coli. | |||||||||
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | |||||||||
| Formulation | GM-CSF Mouse was lyophilized with no additives. | |||||||||
| Solubility | It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 20mM AcOH (acetic Acid) not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | |||||||||
| Stability | Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GM-CSF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
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| Purity | Greater than 98.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
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| Amino acid sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Arg. | |||||||||
| Biological Activity | The ED50 as determined by the dose-dependant stimulation of the proliferation of murine FDC-P1 cell line is < 0.2 ng/ml, corresponding to a Specific Activity of 5,000,000 IU/mg. | |||||||||
| Protein content | GM-CSF quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.765 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of GM-CSF as a Reference Standard. |
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| References | 1. Title:Multigene/multisubtype HIV-1 vaccine induces potent cellular and humoral immune responses by needle-free intradermal delivery. Publication: Mol Ther. 2005 Dec;12(6):1197-205. Epub 2005 Aug 22. PMID: 16112909 Applications: The Mouse GM-CSF used for 2 purposes: 1. As an adjuvant for DNA vaccine which included seven plasmids encoding nine HIV-1 proteins. The mice were injected with the DNA vaccine together with recombinant mouse GM-CSF. 2. Used in Elisa. 2. Title: Neospora caninum: cloning and Publication: Exp Parasitol. 2010 Aug;125(4):357-62. Epub 2010 Mar 6. PMID: 20211619 Applications: Used for immunoblotting 3. Title: Intranasal Granulocyte-Macrophage Colony-Stimulating Factor Reduces the Aspergillus Burden in an Immunosuppressed Murine Model of Pulmonary Aspergillosis. Publication: Antimicrob Agents Chemother. 2008 February; 52(2): 716–718. Published online 2007 November 5 Applications: GM-CSF as tested a therapeutic potential in a murine model of pulmonary aspergillosis. In summary, this pilot study indicates that GM-CSF administered intranasally may be a novel therapeutic approach for the prevention or treatment of pulmonary fungal infections and may augment the efficacies of antifungal agents. GM-CSF was given intranasal. 4. Title: Dendritic Cell-Based Therapeutic Vaccination against Myeloma: Vaccine Formulation Determines Efficacy against Light Chain Myeloma Publication: The Journal of Immunology February 1, 2009 vol. 182 no. 3 1667-1673 http://www.jimmunol.org/content/182/3/1667.full |
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| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. | |||||||||
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